ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (5): 768-773.doi: 10.11843/j.issn.0366-6964.2015.05.012

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Effects of β-conglycinin Hydrolyzed Peptide on the Permeability and Expression of Tight Junction Protein in Piglet Intestinal Epithelial Cells

LIU Dan-dan,ZHAO Yuan*,HAN Rui,ZHANG Shi-yao   

  1. (Key Laboratory of Animal Production,Product Quality and Security of Ministry of Education,Key Laboratory of Animal Nutrition and Feeds Science in Jilin Province,College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China)
  • Received:2014-07-08 Online:2015-05-23 Published:2015-05-23

Abstract:

The objective of this study was to evaluate the effects of β-Conglycinin hydrolyzed peptide(52 ku) on the permeability and expression of tight junction protein Occludin and ZO-1 in piglet intestinal epithelial cells(IPEC),which would provide a basis for exploring the mechanisms of intestinal damage.IPEC were treated with different concentrations(0,0.125,0.25,0.5,1 and 2 mg•mL-1) hydrolyzed peptide for 2,4,6,8,12,24 h.The effects of hydrolyzed peptide(52 ku) on cell membrane permeability were determined by measuring the cell viability(MTT values),transepithelial resistance values(TEER) and alkaline phosphatase(AP) activity in cell culture medium.The mRNA expression of Occludin and ZO-1 were detected by real-time PCR after IPEC treated with 0.5 mg•mL-1 hydrolyzed peptide for 24 h.The results showed that MTT and TEER of intestinal epithelial cells treated with hydrolyzed peptide(52 ku) with 0.5 mg•mL-1 were decreased by a time-dose dependent manner(P<0.05).With the increase concentration of hydrolyzed peptide(52 ku),AP activity in 24 h treatment group had a significant increased trend(P<0.05) by dose dependent manner.Also,hydrolyzed peptide(52 ku) could decrease the relative expression of Occludin and ZO-1 mRNA in IPEC(P<0.05).In conclusion,hydrolyzed peptide(52 ku) destructed the permeability and reduced Occludin or ZO-1 mRNA expression of tight junction protein in IPEC.

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